Potassium Phosphate, Tribasic → Tricalcium Phosphate

Participants

Reaction Steps

A sterile calcium phosphate suspension was prepared by sequentially passing 100 ml of 10% calcium chloride and 50 ml of 10% dibasic potassium phosphate through a microfilter having a pore size of 0.45 μm. The filtrate was directed into a sterile container and a calcium phosphate suspension resulted. The suspension was adjusted to a pH of 7.4 with 10N sodium hydroxide, and 15 ml of the suspension was thereafter added to an additional 100 ml of the medium prepared earlier, that had been held at 50° C., to form a calcium phosphate-containing overlayer agar. The pH of this overlayer agar was aseptically adjusted to 7.4, by adding, when necessary, either 1N sodium hydroxide or 1N hydrochloric acid. 5 mls of the overlayer agar were then disposed on the free surface of the solidified basal layer of each petrie dish so prepared, and the resulting overlayer agar coating was allowed to solidify. The plates thus prepared were dried prior to their use by incubation for 24 hours at 37° C.

Provenance & Evidence