Sodium Bicarbonate → HCID 15159823, compound

Participants

Reaction Steps

Boc-tryptophyl-leucyl-β-alanyl-diazomethane (Section 5.7.1) (0.24 g, 0.47 mmol) was dissolved in ethyl acetate (50 ml). Ether/HCl (40 ml) was added followed by 5% NaHCO3 (60 ml) 1 minute later. The mixture was agitated to neutralise the acid and the aqueous layer was removed. The organic phase was dried over magnesium sulphate and reduced to dryness. The peptide was recrystallised from ethyl acetate/petrol ether (40°/60° C.) and then diethyl ether/petrol ether (40°/60° C.). Yield 0.2 g (82%), m.p. 79°-81° C. The product was homogeneous as judged by tlc in chloroform/methanol (4:1) (Rf 0.770) or chloroform/methanol (1:1) (Rf 0.777). (Found: C, 59.83; H, 7.35; N, 10.81. C26H37O5N4Cl1 requires C, 60.00; H, 7.11; N, 10.77%).

Provenance & Evidence