HuagongsheHuagongshe Chemistry Print Report · For research reference only
Acetonitrile → 4,5-Diaminofluorescein diacetate
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Acetonitrile | HCID 6342 | — |
| Reactant | Acetic Anhydride | HCID 7918 | — |
| Reactant | Cesium carbonate | HCID 10796 | — |
| Reactant | 4,5-Diaminofluorescein | HCID 10666340 | — |
| Product | 4,5-Diaminofluorescein diacetate | HCID 6603693 | — |
Reaction Steps
DAF-2 obtained in Example 4 was dissolved in acetonitrile containing cesium carbonate, and the solution was added with 1 equivalent of acetic anhydride and stirred at room temperature for one hour. After evaporating the solvent under reduced pressure, the residue was purified by silica gel column chromatography to obtain DAF-2 DA.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US05874590 |
| Source dataset | uspto-grants-1999_02 |
| ORD ID | ord-d8d3631a1f674387bbb1c435b520f954 |
| Source note | uspto-grants-1999_02 |