huagongshe.com
HRID37079

反应详情

EQUATION

反应方程式

HRID 37079 的结构方程式

REACTANTS

反应物

2
结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
HCID60961

Adenosine

C10H13N5O4

结构：Nc1ncnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1F
HCID100253

(2R,3R,4R,5R)-5-(6-Aminopurin-9-yl)-4-fluoro-2-(hydroxymethyl)oxolan-3-ol

C10H12FN5O3

PRODUCTS

生成物

1
结构：O=c1[nH]cnc2c1ncn2[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1F
HCID135435172

2'-Deoxy-2'-fluoroinosine

C10H11FN4O4

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O
溶剂HCID962

Water

H2O

PROCEDURE

实验过程

6-Amino-9-(2-deoxy-2-fluoro-β-D-ribofuranosyl)-9H-purine (0.29 g, 1.0 mmole), prepared as in Example 8, was dissolved in 100 ml of water. Calf intestinal adenosine deaminase (4 I.U., Boehringer Mannheim) was added and the solution incubated at 37° C. for 24 hrs. The solvent was removed under vacuum. The residue was dissolved in water/n-propanol (7/3) and chromatographed on a 5×90 cm column of Sephadex G-10 (Pharmacia LKB) with water/n-propanol (7/3) as the solvent. Product-containing fractions were combined and the solvent removed under vacuum. The residue was dissolved in water and lyophilized to obtain title compound that analyzed as a hydrate.

WORKUP

后处理

  1. customThe solvent was removed under vacuum
  2. dissolutionThe residue was dissolved in water/n-propanol (7/3)
  3. customchromatographed on a 5×90 cm column of Sephadex
  4. customthe solvent removed under vacuum
  5. dissolutionThe residue was dissolved in water