Water → D-(-)-Fructose

Participants

Reaction Conditions

Reaction Steps

A water-insoluble tannin preparation [i.e. chinese gallotannin covalently bound to cellulose through the linkage --CH2CH(OH)CH2NH--(CH2)6 --NHCH2CH(OH)CH2 --] is prepared in the same manner as described in Example 1-(2). 25 mg of glucose isomerase (total activity: 690 units) dissolved in 20 ml of an aqueous 0.1 M sodium chloride solution are added to 500 mg (wet form) of the water-insoluble tannin preparation, and the mixture is stirred at 25° C for 20 minutes. Then, the precipitates are collected by filtration and washed with water. 500 mg (wet form) of an immobilized glucose isomerase preparation [i.e. glucose isomerase adsorbed by the water-insoluble tannin preparation] are thereby obtained. It shows an isomerase activity of 326 units. [One unit of glucose isomerase is defined as the enzymatic activity which affords one mg of fructose by reaction with an aqueous 3.6% glucose solution at 70° C for 1 hour in the presence of 0.025 M magnesium sulfate].

Provenance & Evidence