Sucrose → D-(-)-Fructose

Participants

Reaction Steps

The experiment of Example 1 was repeated, except that the resins were washed with increasing volumes (2, 4, 6, or 8 bed volumes) of a dilute (4 mM) solution of NaOH. Fructose retention on the column was extended, but fructose still eluted as a distinct peak within reasonable times (at about 4.5, 5, 5.5, and 6 minutes, respectively). By contrast, retention of myo-inositol was not affected, and still left the column at about 3 minutes, as was observed in Example 1. Results for other mono-and di-saccharides that have been tested in comparable experiments (to date, glucose and sucrose) were similar to the results for fructose.

Provenance & Evidence