Borate → Biotin

Participants

Reaction Steps

Biotinylpentylamine (0.001 mol) and isothiocyanatobenzyl-diethylenetriamine-pentaacetic acid (ITC-DTPA, 0.001 mol) are mixed together in borate buffer, 0.1M, pH 8, and stirred for 3 hours at room temperature. The biotin-DTPA is purified on a C-18 reverse-phase HPLC column run in an acetonitrile/water gradient. The acetonitrile is removed under reduced pressure on a rotary evaporator and the water by lyophilization to give a white powdery product. The structure, biotinylpentylamino-thioureaylbenzyl-DTPA (biotin-DTPA) is confirmed by mass spectral and micro analysis.

Provenance & Evidence