Hydrochloric Acid → (S)-4-(4-Amidinobenzoylamino)acetyl-3-[3-(4-amidinobenzoylamino)propyl]-2-oxopiperazine-1-acetic acid hydrochloride

Participants

Reaction Conditions

Reaction Steps

In 5 ml of 0.5N hydrochloric acid was dissolved 1 g of (S)-4-(4-amidinobenzoylamino)acetyl-3-[3-(4-amidinobenzoylamino)propyl]-2-oxopiperazine-1-acetic acid trifluoroacetate produced in Working Example 2. The solution was stirred for 5 minutes at 0° C., which was allowed to be adsorbed on a CHP-20 column. The column was washed with water until the eluate showed neutral pH. The column was then subjected to elution with 10% acetonitrile/water. Fractions of the eluate were combined and freeze-dried to afford 0.7 g of the titled compound as a colorless amorphous powdery product.

Provenance & Evidence