反应详情

温度

120 °C

Borane-pyridine (available from Aldrich) is added to a suspension of 2-(4-nitrobenzyl)-3,6,9,12-tetraoxo-1,4,7,10-tetraazacyclododecane in pyridine. The mixture is heated under reflux at 120° C. for 14 hours. The solvent is removed under reduced pressure. The residue is taken up in methanol and heated under reflux for 2 hours. The solvent is removed under reduced pressure. The residue is dissolved in methanol and re-evaporated. The solid residue is purified by chromatography on reverse phase Baker G18 silica gel available from J.T. Baker, Inc., Phillipsburg, N.J. Conversion of this product to nitro-benzyl-DOTA is accomplished via the procedure discussed in Renn et al., Bioconj. Chem., 3:563-569, 1992 or as set forth is subpart A above.

1. temperatureThe mixture is heated
2. customThe solvent is removed under reduced pressure
3. temperatureheated
4. temperatureunder reflux for 2 hours
5. customThe solvent is removed under reduced pressure
6. dissolutionThe residue is dissolved in methanol
7. customre-evaporated
8. customThe solid residue is purified by chromatography on reverse phase Baker G18 silica gel available from J.T