Hydrochloric Acid → HCID 131721668, compound

Participants

Reaction Steps

Urea (4 g) and ethyl β-[[2-[[(3-aminophenyl)-carbony]amino]acetyl]amino]pyridine-3-propanoate trifluoroacetate salt (4 g) were dissolved in 20% aqueous HCl (50 ml) and refluxed for 6 hours. The reaction was made basic with KOH (pH>12). After 4 hours the reaction was acidified with TFA and purified by HPLC (RP--CH3CN/H2O). The white solid was dissolved in 1:1 CH3CN:H2O (100 ml) and subjected to the resin exchange described in Example 43, Step C. Lyophilization gave the desired product (3.2 g). MS, 1H-NMR and CHNCl were consistent with the desired product.

Provenance & Evidence