HuagongsheHuagongshe Chemistry Print Report · For research reference only
Dicyclohexylcarbodiimide → N-lysyl-5'-O-palmitoyl-deoxycytidine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Dicyclohexylcarbodiimide | HCID 10868 | — |
| Reactant | 5'-O-palmitoyldeoxycytidine | HCID 15484389 | — |
| Reactant | Di-tert-butoxycarbonyl-lysine | HCID 22792075 | — |
| Solvent | Dimethylacetamide | HCID 31374 | — |
| Product | N-lysyl-5'-O-palmitoyl-deoxycytidine | HCID 15484390 | — |
Reaction Conditions
| Temperature | 4 °C |
|---|
Reaction Steps
14 grams of 5′-O-palmitoyl-deoxycytidine is dissolved in 100 ml dimethylacetamide, 1 molar equivalent of di-tert-butoxycarbonyl-lysine is added, and the mixture is cooled in an ice bath. 1.2 molar equivalents (7.4 g) of dicyclohexylcarbodiimide are added and the mixture is stirred at 4° C. for 90 hours. The precipitate (dicyclohexylurea) is removed by filtration. 100 ml of water is added to the filtrate, followed by 1 liter of ethyl acetate.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06297222B1 |
| Source dataset | uspto-grants-2001_10 |
| ORD ID | ord-207a4705b1e24a71808aaae7ffaa6e3f |
| Source note | uspto-grants-2001_10 |