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HRID381215

反应详情

EQUATION

反应方程式

HRID 381215 的结构方程式

REACTANTS

反应物

2
反应SMILES：O
HCID962

Water

H2O

反应SMILES：CCCCCCCCCCCCC/C=C/[C@@H](O)[C@H](CO[C@@H]1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H]3NC(C)=O)[C@H](O[C@]3(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O3)[C@H]2O)[C@H](O)[C@H]1O)NC(=O)CCCCCCCCCCCCCCCCC
HCID9963963

未命名化合物

PRODUCTS

产物

1
反应SMILES：CCCCCCCCCCCCCCC#CC#CCCCC(=O)O
HCID5052279

5,7-DOCOSADIYNOIC ACID

C22H36O2

PROCEDURE

实验过程

Ganglioside GM1, cholera toxin from Vibrio Cholerae, human serum albumin, and wheat germ agglutinin were purchased from Sigma. 5,7-docosadiynoic acid was synthesized. Deionized water was obtained by passing distilled water through a Millipore μF ultrapurification train. Solvents used were reagent grade. The ganglioside GM1 was mixed at 5 mol % with the diacetylene “matrix lipid” monomers. Liposomes were prepared using the probe sonication method and polymerized by UV irradiation (254 nm). The conjugated ene-yne backbone of polydiacetylene liposomes results in the appearance of a deep blue/purple solution. The visible absorption spectrum of the freshly prepared purple liposomes is shown in FIG. 25.

WORKUP

后处理

  1. distillationby passing distilled water through a Millipore μF ultrapurification train
  2. additionThe ganglioside GM1 was mixed at 5 mol % with the diacetylene “matrix
  3. customLiposomes were prepared
  4. customthe probe sonication method
  5. custompolymerized by UV irradiation (254 nm)