反应详情

S-(2,3-dihydroxypropyl)cysteine (2.45 g, 12.6 mmole) was dissolved in 9% sodium carbonate (20 ml). A solution of fluorenylmethoxycarbonyl-N-hydroxysuccinimide (3.45 g, 10.5 mmole) in acetonitrile (20 ml) was added and the mixture stirred for 2 h, then diluted with water (240 ml), and extracted with diethyl ether (25 ml×3). The aqueous phase was acidified to pH 2 with concentrated hydrochloric acid and was then extracted with ethyl acetate (70 ml×3). The extract was washed with water (50 ml×2) and saturated sodium chloride solution (50 ml×2), dried over sodium sulfate and evaporated to dryness. Recrystallisation from ether and ethyl acetate at −20° C. yielded a colourless powder (2.8 g, 6.7 mmole, 63.8%).

1. extractionextracted with diethyl ether (25 ml×3)
2. extractionwas then extracted with ethyl acetate (70 ml×3)
3. washThe extract was washed with water (50 ml×2) and saturated sodium chloride solution (50 ml×2)
4. dry with materialdried over sodium sulfate
5. customevaporated to dryness
6. customRecrystallisation from ether and ethyl acetate at −20° C.