Sodium Hydroxide → HCID 66660016, compound

Participants

Reaction Steps

To the solution of methyl cis-5-[(tert-butoxycarbonyl)amino]-1-[4-methyl-6-(1H-pyrazol-5-ylamino)pyridin-2-yl]piperidine-3-carboxylate (11.0 mg) in MeOH (5 ml) was added 5 M aqueous solution of NaOH (468.0 μl) at room temperature. After stirring for 4 hours, NH4Cl was added for neutralization and the mixture was concentrated. The residue was suspended into the mixed solvent of CHCl3 and MeOH. The insoluble material was removed by filtration. The filtrate was concentrated and the residue was purified with preparative RP-HPLC. The desired fraction was neutralized by addition of aqueous solution of NH3 and concentrated. And the residue was purified again with preparative RP-HPLC (mobile phase: H2O and acetonitlile, without TFA) to give cis-5-[(tert-butoxycarbonyl)amino]-1-[4-methyl-6-(1H-pyrazol-5-ylamino)pyridin-2-yl]piperidine-3-carboxylic acid (1.82 mg) as colorless solid.

Provenance & Evidence