Ammonia → HCID 57474695, compound

Participants

Reaction Steps

2-(2-Hydroxyethylamino)-9-(2-methoxyphenyl)-8-oxo-8,9-dihydro-7H-purine-6-carboxamide Ethyl 2-(2-hydroxyethylamino)-9-(2-methoxyphenyl)-8-oxo-8,9-dihydro-7H-purine-6-carboxylate (0.400 g) and ammonia gas in methanol (10 mL) were reacted according to General Procedure G and purified using reverse-phase preparative HPLC (5-60% acetonitrile+0.1% TFA in H2O+0.1% TFA, over 30 min) to afford the title compound (0.015 g, 8%). 1H NMR (400 MHz, DMSO-d6) δ 10.49 (s, 1H), 7.86 (s, 1H), 7.81 (s, 1H), 7.48 (t, J=6.79, 1H), 7.36 (d, J=7.99, 1H), 7.20 (d, J=8.39, 1H), 7.07 (t, J=7.59, 1H), 6.78 (s, 1H), 4.50 (t, J=5.59, 1H), 3.72 (s, 3H), 3.44 (q, J=6.39, 2H), 3.31 (m, 2H); MS (ESI) m/z 345.2 [M+1]+; mp 157-160° C.

Provenance & Evidence