Ammonia → HCID 5271807, compound

Participants

Reaction Steps

In 4 mL of methanol was dissolved 0.26 g of methyl 6-chloro-4-[(2R,3R,4S,5S)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-3-oxo-3,4-dihydro-2-pyrazinecarboxylate. While cooling the solution with ice, gaseous ammonia was introduced until saturation. The reaction mixture was stirred at an ice-cooled temperature for one hour and then the solvent was removed under reduced pressure. By purifying the residue thus obtained by column chromatography [eluent: chloroform:methanol=7:1], 0.06 g of 6-chloro-4-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-3-oxo-3,4-dihydro-2-pyrazinecarboxamide was obtained as a light yellow-colored solid product.

Provenance & Evidence