HuagongsheHuagongshe Chemistry Print Report · For research reference only
Trifluoroacetic acid → Fmoc-Lys(biotinyl)-OH
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Trifluoroacetic acid | HCID 6422 | — |
| Reactant | 1-Hydroxybenzotriazole | HCID 75771 | — |
| Reactant | Diisopropylethylamine | HCID 81531 | — |
| Reactant | Silane, tris(1-methylethyl)- | HCID 138754 | — |
| Reactant | Biotin | HCID 171548 | — |
| Reactant | (2S)-6-amino-2-({[(9H-fluoren-9-yl)methoxy]carbonyl}amino)hexanoic acid | HCID 7010557 | — |
| Solvent | Dichloromethane | HCID 6344 | — |
| Solvent | N-methyl-2-pyrrolidone | HCID 13387 | — |
| Product | Fmoc-Lys(biotinyl)-OH | HCID 11039242 | — |
Reaction Steps
Fmoc-Lys (mtt)-Resin was stirred with a mixture of 1% trifluoroacetic acid (TFA) and 0.1% triisopropylsilane (TIS) in DCM at 0° C. for 30 min and then for one hour at room temperature. Then, the resin was washed with NMP and DCM, and dried in vacuum. 2.68 gr. of loaded resin were obtained. This resin was then swelled with 25 ml NMP in the Presence of 930 mg Biotin, 1.44 gr. O-benzotriazole-N,N,N′,N′-tetramethyl-uronium-hexafluophosphate (HBTU), 513 mg N-hydroxybenzotriazole (HOBt) and 1 ml of diisopropylethylamine (DIEA). The reaction was stirred for 4.5 hr. Then, the resin was washed with NMP and DCM, and dried in vacuum. 2.8 gr. of Fmoc-Lys(Biotin)-resin were obtained.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06602852B1 |
| Source dataset | uspto-grants-2003_08 |
| ORD ID | ord-22dad0b7c3cc4d3faa07e200c8ef0318 |
| Source note | uspto-grants-2003_08 |