反应详情

Streptomyces rimosus subsp. rimosus ATCC10970 maintained on a quarter strength ATCC172 agar slope was inoculated as a loopful of spores into a 300 ml Erlenmeyer flask containing 50 ml of AS-7H inoculum medium. This was allowed to incubate for 2 days at 28° C., 200 rpm on an Infors Multitron™ Shaker with 1″ throw. 2 mls of this inoculum medium was then transferred to twenty six 300 ml Erlenmeyer flask containing 50 ml of AP-5H production medium and incubated under the same conditions for a further 24 hours. At this point 4.5 mg of N-[6-methoxy-5-(2-methoxyphenoxy)-2-(2-pyrimidinyl)-4-pyrimidinyl]-3,3-dimethyl-1-butanesulfonamide (Preparation 1) dissolved in 0.5 ml of methanol was added to each of the flasks and the fermentation allowed to continue under the same conditions for a further 20 hours. The flask was then extracted with 200 mls of ethyl acetate and the combined ethyl acetate solubles concentrated to dryness to give a gum solid, 430 mg .

1. temperaturerimosus ATCC10970 maintained on a quarter strength ATCC172 agar slope
2. additioncontaining 50 ml of AS-7H inoculum medium
3. additioncontaining 50 ml of AP-5H production medium
4. waitincubated under the same conditions for a further 24 hours
5. additionwas added to each of the flasks
6. waitto continue under the same conditions for a further 20 hours
7. extractionThe flask was then extracted with 200 mls of ethyl acetate
8. concentrationthe combined ethyl acetate solubles concentrated to dryness
9. customto give a gum solid, 430 mg