Water → 6-tert-Butyl-8-fluoro-2-(2-hydroxymethyl-3-{2-[5-(morpholine-4-carbonyl)-pyridin-2-ylamino]-pyridin-4-yl}-phenyl)-2H-phthalazin-1-one

Participants

Reaction Steps

Acetic acid 2-(6-tert-butyl-8-fluoro-1-oxo-1H-phthalazin-2-yl)-6-{2-[5-(morpholine-4-carbonyl)-pyridin-2-ylamino]-pyridin-4-yl}-benzyl ester (200 mg, 0.31 mmol) was dissolved in methanol (10 ml). K2CO3 (86 mg, 0.62 mmol) was added at room temperature, and the mixture was stirred at that temperature for 2 hours. The mixture was poured into water (10 ml), extracted with DCM (100 ml) and then washed with saturated aqueous solution of sodium chloride (100 ml). The combined organic extract was dried over sodium sulfate and concentrated. The residue was purified by silica gel column (petroleum ether:ethyl acetate=1:2). The desired product was obtained as a white solid (130 mg, yield 69%). 1H NMR (300 MHz, MeOD): δ 8.36 (d, J=2.6 Hz, 1H), 8.22-8.18 (m, 2H), 7.73 (d, J=1.5 Hz, 2H), 7.65-7.60 (m, 2H), 7.57-7.51 (m, 1H), 7.50-7.46 (m, 1H), 7.38 (d, J=7.6 Hz, 2H), 7.00 (dd, J=5.2, 1.5 Hz, 1H), 4.36 (s, 2H), 3.59 (s, 8H), 1.36 (d, J=5.7 Hz, 9H). LC-MS: 609[M+1]+, tR=1.407 min. HPLC: 97.79% at 214 nm, 99.31% at 254 nm, tR=3.479 min.

Provenance & Evidence