反应详情

An amount of 400 mg (0.70 mmol) of the compound from example 7A was introduced in 17 ml of acetic acid and then admixed cautiously with 8.6 ml of water. The batch was stirred at RT for 12 hours. After the reaction mixture had been concentrated on a rotary evaporator, the residue was purified directly by preparative HPLC (column: YMC GEL ODS-AQ S-5/15 μm; eluent gradient: acetonitrile/water 10:90→95:5). Removal of the solvent on a rotary evaporator gave the product as a white solid.

1. concentrationAfter the reaction mixture had been concentrated on a rotary evaporator
2. customthe residue was purified directly by preparative HPLC (column: YMC GEL ODS-AQ S-5/15 μm; eluent gradient: acetonitrile/water 10:90→95:5)
3. customRemoval of the solvent
4. customon a rotary evaporator