反应详情

The activity of α-isomaltosyl glucosaccharide-forming enzyme was measured by the following assay: A substrate solution was prepared by dissolving maltotriose in 100 mM Glycine-NaOH buffer (pH 8.4) to give a concentration of 2% (w/v). A reaction mixture was prepared by mixing 0.5 ml of the substrate solution and 0.5 ml of an enzyme solution, and incubated at 40° C. for 60 minutes. After stopping the reaction by boiling for 10 minutes, the amount of maltose formed in the reaction mixture was determined by high-performance liquid chromatography (HPLC). One unit of α-isomaltosyl glucosaccharide-forming activity was defined as the amount of the enzyme that forms one μmole of maltose per minute under the above conditions. HPLC was carried out using “SHODEX KS-801”, a column commercialized by Showa Denko K.K., Tokyo, Japan, at a column temperature of 60° C. and a flow rate of 0.5 ml/minutes of water, and using “RI-8012”, a differential refractometer commercialized by Tosoh Corporation, Tokyo, Japan.

1. customA substrate solution was prepared
2. customto give a concentration of 2% (w/v)
3. customA reaction mixture was prepared
4. customthe reaction
5. waitby boiling for 10 minutes