反应详情

To a solution of 2-((4-(4-(3-cyclobutylureido)benzoyl)piperazin-1-yl)methyl)-oxazole-4-carboxylic acid 2,2,2-trifluoroacetate (8 mg, 15 μmol) in N,N-dimethyl-acetamide (1 mL) was added O-(7-azabenzotriazol-1-yl)-N,N,N,N-tetramethyl uronium hexafluorophosphate (5.7 mg, 154 μmol; HATU), tert-butylamine (1.6 μL, 15 μmol) and N,N-diisopropylethylamine (3.9 μL, 23 μmol). The reaction mixture was stirred at room temperature for 20 hours and was then concentrated under vacuum. The crude residue was purified by acidic reverse phase HPLC to give the title compound (5.6 mg). MS (ESI) m/z 483.1 [M+H]+

1. concentrationwas then concentrated under vacuum
2. customThe crude residue was purified by acidic reverse phase HPLC