反应详情

tert-Butyl 4-(4-(3-cyclobutylureido)-3-fluorobenzoyl)piperazine-1-carboxylate (2.8 g, 66.67 mmol), trifluoracetic acid and dichloromethane were stirred for 20 hours then concentrated under reduced pressure. The residue was taken up in dichloromethane/methanol, loaded on to a strong cation exchange column and washed with dichloromethane/methanol. Elution of the column with 2M ammonia in methanol gave the title compound (1.6 g). MS (ESI) m/z: 321.4 [M+H]+

1. concentrationthen concentrated under reduced pressure
2. washwashed with dichloromethane/methanol
3. washElution of the column with 2M ammonia in methanol