Hydrochloric Acid → HCID 20583550, compound

Participants

Reaction Steps

A solution of 47 mg (0.063 mmol) of N-[(5-(3,5 -dichlorobenzensulfonyl)-1-ethyl-1,4,5,6-tetrahydropyrrolo[3,4-c]pyrazole-4 (S)-carbonyl]-4-(3′,5′-dichloroisonicotinoyl)amino-(L)-phenylalanine, methyl ester in 2 mL of MeOH and 0.5 mL of water was treated with 7 mg (0.167 mmol) of LiOH—H2O. After stirring for 2 hr the solution was acidified (pH 2) with 1.2 N HCl and concentrated in vacuo. The residue purified by preparative TLC on silica eluted with 94.5:5:0.5 CH2Cl2:MeOH:HOAc to yield N-[(5-(3,5-dichlorobenzensulfonyl)-1 -ethyl-1,4,5,6-tetrahydropyrrolo[3,4-c]pyrazole-4(S)-carbonyl]-4-(3′,5′-dichloroisonicotinoyl)amino-(L)-phenylalanine (35 mg) as a solid. LC-MS: m/e 727 (M+1).

Provenance & Evidence