反应详情

N5-(1H-Benzo[d]imidazol-2-yl)-N4-(2-methyl-4-(piperazin-1-yl)phenyl)-1H-imidazole-4,5-dicarboxamide (1 equivalent) and dichloromethane were stirred for 5 minutes. Phenyl isocyanate (1.5 equiv) was added and the reaction mixture was stirred at room temperature for 16 hours. The reaction mixture was evaporated under reduced pressure. The resulting crude product was purified by reverse phase HPLC (aqueous ammonium acetate/acetonitrile to give N5-1H-benzimidazol-2-yl-N4-(2-methyl-4-{4-[(phenylamino)carbonyl]piperazin-1-yl}phenyl)-1H-imidazole-4,5-dicarboxamide. 1H-NMR (400 MHz, DMSO-d6): δ 8.86 (s, br, 1H), 8.38 (s, br, 1H), 7.67 (m, 2H), 7.52 (m, 3H), 7.43 (m, 3H), 7.26 (m, 2H), 6.97 (m, 1H), 3.84 (s, br, 4H), 3.41 (s, br, 4H), 2.33 (s, 3H). MS (EI): 564 (MH+).

1. customThe reaction mixture was evaporated under reduced pressure
2. customThe resulting crude product was purified by reverse phase HPLC (aqueous ammonium acetate/acetonitrile