Triethylamine → rac-(2'S,3'R,4'S,5'R)-6-chloro-4'-(3-chloro-2-fluoro-phenyl)-2'-(2,2-dimethyl-propyl)-2-oxo-1,2-dihydro-spiro[indole-3,3'-pyrrolidine]-5'-carboxylic acid (4-carbamoyl-3-methoxy-phenyl)-amide

Participants

Reaction Conditions

Reaction Steps

To a solution of rac-4-{[(2′S,3′R,4′S,5′R)-6-chloro-4′-(3-chloro-2-fluoro-phenyl)-2′-(2,2-dimethyl-propyl)-2-oxo-1,2-dihydro-spiro[indole-3,3′-pyrrolidine]-5′-carbonyl]-amino}-2-methoxy-benzoic acid prepared in Example 16 (55 mg, 0.09 mmol) in N,N-dimethylformamide (2 mL) was added EDCI (34 mg, 0.18 mmol), HOBt (24 mg, 0.18 mmol), NH4Cl (48 mg, 0.9 mmol), and triethylamine (18 mg, 0.18 mmol) sequentially. The reaction mixture was heated at 80° C. for 1 h. The mixture was poured into water, then extracted with ethyl acetate three times. The organic layers were combined, washed with water, brine, dried over MgSO4, and concentrated. The residue was purified by chromatography (25% to 100% EtOAc in hexanes) to give rac-(2′S,3′R,4′S,5′R)-6-chloro-4′-(3-chloro-2-fluoro-phenyl)-2′-(2,2-dimethyl-propyl)-2-oxo-1,2-dihydro-spiro[indole-3,3′-pyrrolidine]-5′-carboxylic acid (4-carbamoyl-3-methoxy-phenyl)-amide as a off white solid (Yield, 35 mg, 64%)

Provenance & Evidence