反应详情

A dimethyl sulfoxide solution (1.0 ml) of 5-[8-chloro-9-(cyclopropylmethyl)-6-morpholin-4-yl-9H-purin-2-yl]pyrimidin-2-amine (75.3 mg, 0.19 mmol) and morpholine (65.8 μl, 0.75 mmol) was heated at 140° C. and stirred for 4 hours. Morpholine (32.9 μl, 0.38 mmol) was added at room temperature and the resulting mixture was further stirred at 140° C. for 2 hours. The resulting mixture was left standing to cool followed by the addition of dichloromethane-methanol (10:1) and the resulting mixture was washed with saturated aqueous sodium hydrogen carbonate solution. The organic layer was dried over anhydrous sodium sulfate, the mixture was filtrated, the filtrate was concentrated under reduced pressure, and the resulting residue was purified by preparative thin layer silica gel chromatography (dichloromethane:methanol=15:1) to give the title compound (85.9 mg, 100%) as a white solid.

1. stirringthe resulting mixture was further stirred at 140° C. for 2 hours
2. waitThe resulting mixture was left
3. temperatureto cool
4. washthe resulting mixture was washed with saturated aqueous sodium hydrogen carbonate solution
5. dry with materialThe organic layer was dried over anhydrous sodium sulfate
6. filtrationthe mixture was filtrated
7. concentrationthe filtrate was concentrated under reduced pressure
8. customthe resulting residue was purified by preparative thin layer silica gel chromatography (dichloromethane:methanol=15:1)