HuagongsheHuagongshe Chemistry Print Report · For research reference only
Potassium Chloride → 2,8-Dibenzyl-3-methoxy-6-phenyl-imidazo[1,2-a]pyrazine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Potassium Chloride | HCID 4873 | — |
| Reactant | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| Reactant | X 100 | HCID 19153 | — |
| Reactant | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | HCID 23831 | — |
| Reactant | Coelenterazine | HCID 135445694 | — |
| Solvent | Glycerol | HCID 753 | — |
| Product | 2,8-Dibenzyl-3-methoxy-6-phenyl-imidazo[1,2-a]pyrazine | HCID 66702735 | — |
Reaction Steps
Chemiluminescence of P450 altered coelenterazine or coelenterazine derivatives was determined immediately following addition of 80 microliter of 37.5 mM Hepes pH7.4; 625 mM KCl; 0.125 mM EDTA pH 8.0; 0.25% Triton X-100; 0.125% Mazu; 1.25% Glycerol; 0.25 mg/mL gelatin to the P450 reactions. Renilla luciferase (1.2 ng/mL) (purchased from Chemicon) was added for detection of bioluminescence of P450 altered coelenterazine or coelenterazine derivatives.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08106052B2 |
| Source dataset | uspto-grants-2012_01 |
| ORD ID | ord-535ac1a8fe8f44c28e3893af634bb01b |
| Source note | uspto-grants-2012_01 |