Trimethylsilyldiazomethane → HCID 14775497, compound

Participants

Reaction Steps

The methyl ester, JR2171, was synthesized by dissolving approximately 10.0 mg of 3-(4-{2-[6-amino-9-(5-ethylcarbamoyl-3,4-dihydroxy-tetrahydro-furan-2-yl)-9H-purin-2-ylamino]-ethyl}-phenyl)-propionic acid (CGS21680) in 5 mL 10% MeOH/CH2Cl2 and adding TMS Diazomethane (2.0 M solution in hexanes) dropwise until a yellow color persisted. After stirring for 30 minutes the solvent was removed under reduced pressure. The crude compound was then purified by HPLC using a C18 column and a MeOH/H2O gradient, yielding 6.7 mg of compound (66% yield). Characterization by HRMS, APCI MS, 1H NMR, and 13C NMR were consistent with the putative structure.

Provenance & Evidence