(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-(-)-Fructose

Participants

Reaction Conditions

Reaction Steps

Streptomyces olivaceus, strain NRRL B-3583, is cultivated in a nutrient medium containing 0.7 percent xylose, 0.3 percent dextrose, 0.5 percent beef extract, 0.25 percent yeast extract, 1.0 percent peptone, 0.5 percent NaCl, 0.05% MgSO4.7H2O and 0.024 percent CoCl2.6H2O. The fermentation is allowed to proceed at 28° C. for 24 hours. A 2 percent (weight per volume) solution of a cationic polyelectrolyte is added to the fermentation broth in amounts equivalent to 0.05 percent (weight per volume) of the dry flocculant. The broth is briefly agitated to promote aggregation of the cells. Agitation is then stopped and sedimentation of the flocculated cells is allowed to proceed. The cell aggregates are harvested in a manner analogous to that described in Example 1. The flocculated cells are dried at 60° C. for 24 hours before subjecting the dried cell mass to gentle mechanical crushing action to reduce to particle sizes suitable for column operation. The crushed cell material is poured into a jacketed column partially filled with a 1.0 M glucose solution. The packed column is heated to 70° C. and a 1.0 M glucose solution containing 0.04 M MgCl2.6H2O adjusted to pH 8 is passed through the column to give conversion of glucose to fructose.

Provenance & Evidence