Water → HCID 21454978, compound

Participants

Reaction Steps

1.0 G. of 3,6-bis(5-chloro-2-piperidinyl)-2,5-piperazinedione is heated in 50 ml. of water for 1/2 hour on a steam bath. The solution is filtered, neutralized with dilute sodium hydroxide and freeze-dried to a white solid weighing 1.16 g. Purification of 300 mg. is accomplished by chromatography on 6 × 1000 μ on silica gel plate using 5:1:2 n-butanol:acetic acid:water as an eluant to give 165 mg. which is dissolved in 15 ml. of hot methanol. Filter solution and bubble in hydrogen chloride gas. Collect precipitate and wash 2 × 5 ml. with methanol and 20 ml. of ether to give 80 mg. of 3,6-bis-(5-hydroxy-2-piperidinyl)-2,5-piperazinedione dihydrochloride.

Provenance & Evidence