HCID 178489550, compound → HCID 178489549, compound

Participants

Reaction Steps

Dinucleoside monophosphate 3 (20 mg; 20 μmol) was dissolved in 2 mL of 1:1 CH3OH-t-butylamine. The reaction mixture was maintained at 40°-45° C. for 2 days, then concentrated under diminished pressure. The residue was purified by preparative silica gel TLC; development was with 9:1 CH2Cl2 -CH3OH. Dinucleoside monophosphate 4 was isolated as an off-white gummy solid following lyophilization, yield 15 mg (84%); silica gel TLC Rf 0.50 (10:1 CH2Cl2 -CH3OH); 1H-NMR (CDCl3, (CH3)4Si) & 0.8-0.9 (m), 1.2-1.35 (m), 1.32 (s), 1.81 (s), 2.1-2.9 (m), 3.00 (m), 3.4-4.3 (m), 3.71 (s), 4.4 (m), 4.6 (m), 5.04 (m), 6.07 (t), 6.35 (m), 6.78 (d), 7.2-7.4 (m), 7.50 (s) and 8.46 (br s).

Provenance & Evidence