Cefoperazone sodium → Cefoperazone

HRID 442621Notes

Reaction equation

HRID 442621 reaction equation
[Na].C[C@@H]1C[C@@H]([C@H](O)CC2CC(=O)NC(=O)C2)C(=O)[C@@H](C)C1.[CH3:22][CH2:23][N:24]1[C:30](=[O:31])[C:28](=[O:29])[N:27]([C:32]([NH:34][C@@H:35]([C:43]([NH:45][C@@H:46]2[C:49](=[O:50])[N:48]3[C:51]([C:63]([O-:65])=[O:64])=[C:52]([CH2:55][S:56][C:57]4[N:61]([CH3:62])[N:60]=[N:59][N:58]=4)[CH2:53][S:54][C@H:47]23)=[O:44])[C:36]2[CH:41]=[CH:40][C:39]([OH:42])=[CH:38][CH:37]=2)=[O:33])[CH2:26][CH2:25]1.[Na+]>CO>[CH3:22][CH2:23][N:24]1[C:30](=[O:31])[C:28](=[O:29])[N:27]([C:32]([NH:34][C@@H:35]([C:43]([NH:45][C@@H:46]2[C:49](=[O:50])[N:48]3[C:51]([C:63]([OH:65])=[O:64])=[C:52]([CH2:55][S:56][C:57]4[N:61]([CH3:62])[N:60]=[N:59][N:58]=4)[CH2:53][S:54][C@H:47]23)=[O:44])[C:36]2[CH:41]=[CH:40][C:39]([OH:42])=[CH:38][CH:37]=2)=[O:33])[CH2:26][CH2:25]1

Participants

4

Reactant 2

HCID 23663974Cefoperazone sodiumHCID 70099272HCID 70099272, compound

Product 1

HCID 44187CefoperazoneYield 10.3%

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent

Procedure

Media: The composition of Campy-Cefex, modified CCDA and Campy-BAP are provided in Table 1. All media were prepared in our laboratories and used immediately or stored in the dark at 4° C. no more than two weeks prior to use. The basal media components were autoclaved at 121° C. for 15 min. The sterilized agar was then tempered in a waterbath to 50°-55° C., and the lysed horse blood and filter-sterilized antibiotics were added and gently mixed before the plates were poured. Filter-sterilized sodium cycloheximide (200 mg/L) was added as a 10% solution in 50% methanol. One gram of sodium cefoperazone was dissolved in 10 ml of sterile, distilled water. Each ml of this solution provided 100 mg cefoperazone/3 L of Campy-Cefex agar. The unused portion of cefoperazone was held in storage at -20° C.

Workup

  1. customare provided in Table 1
  2. customAll media were prepared in our laboratories
  3. customdark at 4° C.
  4. customno more than two weeks
  5. customwas then tempered in a waterbath to 50°-55° C.
  6. additionthe lysed horse blood and filter-sterilized antibiotics were added
  7. additiongently mixed before the plates
  8. additionwere poured
  9. distillationdistilled water

Sources & evidence

ORD record
ord-c124d53991a348728fe66becb41c39f9
Source dataset
uspto-grants-1999_04
Patent number
US05891709

Reaction notes

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