反应详情

Media: The composition of Campy-Cefex, modified CCDA and Campy-BAP are provided in Table 1. All media were prepared in our laboratories and used immediately or stored in the dark at 4° C. no more than two weeks prior to use. The basal media components were autoclaved at 121° C. for 15 min. The sterilized agar was then tempered in a waterbath to 50°-55° C., and the lysed horse blood and filter-sterilized antibiotics were added and gently mixed before the plates were poured. Filter-sterilized sodium cycloheximide (200 mg/L) was added as a 10% solution in 50% methanol. One gram of sodium cefoperazone was dissolved in 10 ml of sterile, distilled water. Each ml of this solution provided 100 mg cefoperazone/3 L of Campy-Cefex agar. The unused portion of cefoperazone was held in storage at -20° C.

1. customare provided in Table 1
2. customAll media were prepared in our laboratories
3. customdark at 4° C.
4. customno more than two weeks
5. customwas then tempered in a waterbath to 50°-55° C.
6. additionthe lysed horse blood and filter-sterilized antibiotics were added
7. additiongently mixed before the plates
8. additionwere poured
9. distillationdistilled water