Water → HCID 178434797, compound

Participants

Reaction Conditions

Reaction Steps

In 20 ml of phosphate buffer (0.2 M, pH 6.4) there was dissolved 0.663 g of 7-[2-(2-imino-4-thiazolin-4-yl)-2-hydroxyimino-acetamido]-3-acetoxymethyl-3-cephem-4-carboxylic acid hydrochloride (syn-isomer) together with 0.198 g of 3-methyl-1,2,4-thiadiazole-5-thiol and 0.252 g of sodium hydrogen carbonate and the mixture was stirred at a temperature of 70° C. for 3.5 hours. The reaction mixture was subjected to column chromatography on polystyrene resin (Amberlite XAD-2, Rohm and Haas Co.), development being carried out with water and 20% ethanol in the order mentioned. The fractions containing the desired product were pooled and lyophilized. By the above procedure there was obtained 0.142 g of the captioned compound.

Provenance & Evidence