Water → HCID 68089376, compound

Participants

Reaction Steps

To 2-{1-[(1-{[(R)-5-(4-cyano-benzyl)-7-(3,5-dichloro-phenyl)-5-methyl-6-oxo-6,7-dihydro-5H-imidazo[1,2-a]imidazole-3-carbonyl]-amino}-cyclopropanecarbonyl)-amino]-cyclopropyl}-isonicotinic acid (30 mg, 0.044 mmol), in DMF (3 mL) was added HATU (50 mg, 0.13 mmol) diisopropylethylamine (23 μL, 0.13 mmol) and mono-Boc-protected ethylenediamine (14 mg, 0.088 mmol). The reaction was shaken at room temperature overnight. Water was added (0.1 mL) and the crude reaction was purified by HPLC to afford {2-[(6-{1-[(1-{[(R)-5-(4-cyano-benzyl)-7-(3,5-dichloro-phenyl)-5-methyl-6-oxo-6,7-dihydro-5H-imidazo[1,2-a]imidazole-3-carbonyl]-amino}-cyclopropanecarbonyl)-amino]-cyclopropyl}-pyridine-3-carbonyl)-amino]-ethyl}-carbamic acid tert-butyl ester. This material was dissolved in dichloromethane (1.0 mL) and 4M HCl in dioxane (0.5 mL) was added. The reaction was shaken for 2 h at room temperature. The volatiles were removed to afford 23 mg of the title compound, m/z 726.3.

Provenance & Evidence