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HRID449779

反应详情

EQUATION

反应方程式

HRID 449779 的结构方程式

REACTANTS

反应物

2
结构：CCO
HCID702

Ethanol

C2H6O

结构：CCCCCCCCCCCCC/C=C/[C@@H](O)[C@H](CO[C@@H]1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H]3NC(C)=O)[C@H](O[C@]3(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O3)[C@H]2O)[C@H](O)[C@H]1O)NC(=O)CCCCCCCCCCCCCCCCC
HCID9963963

未命名化合物

2 次

PRODUCTS

生成物

1
结构：Cc1cc(=O)oc2cc(O)ccc12
HCID5280567

4-Methylumbelliferone

C10H8O3

PROCEDURE

实验过程

The saliva of each experimental group of Test Example 1 in the present specification was used to examine inhibitory activity against cholera toxin binding. 200 μl of an ethanol solution containing 0.1% ganglioside GM1 (w/v) was added to a 96-well plate for ELISA test. Then, the ganglioside GM1 was adsorbed thereonto by air-drying. The saliva of each experimental group was diluted 10 times with PBS containing 1% bovine serum albumin (BSA) and then reacted for 1 hour by the addition of a biotin-conjugated cholera toxin. 100 μl of the reaction solution was added to the plate for ELISA test, and the plate was left for 30 minutes. Then, the supernatant was removed. The plate for ELISA test was washed several times with PBS containing 0.05% Tween 20. Biotin-binding β-galactosidase was added thereto. The plate for ELISA test was left for a given length of time. Then, the supernatant was removed. The plate for ELISA test was washed several times again with PBS containing 0.05% Tween 20, and the contents in the plate were reacted for 30 minutes by the addition of 4-methylumbelliferyl galactose. Then, 4-methylumbelliferone produced was measured with a fluorophotometer (excitation wavelength: 360 nm, measurement wavelength: 460 nm). Then, an inhibition rate was calculated according to the following formula: Inhibition rate(%)={1−(A/B)}×100, wherein

WORKUP

后处理

  1. additionwas added to a 96-well plate for ELISA test
  2. customby air-drying
  3. additionThe saliva of each experimental group was diluted 10 times with PBS
  4. additioncontaining 1% bovine serum albumin (BSA)
  5. customreacted for 1 hour by the addition of a biotin-
  6. customThen, the supernatant was removed
  7. washThe plate for ELISA test was washed several times with PBS
  8. additioncontaining 0.05% Tween 20
  9. additionBiotin-binding β-galactosidase was added
  10. waitThe plate for ELISA test was left for a given length of time
  11. customThen, the supernatant was removed
  12. washThe plate for ELISA test was washed several times again with PBS
  13. additioncontaining 0.05% Tween 20
  14. customthe contents in the plate were reacted for 30 minutes by the addition of 4-methylumbelliferyl galactose