反应详情

温度

0 °C

N-Benzyloxycarbonyl-L-alanine (6.5 g, 28.5 mmol), N,O-dimethylhydroxylamine hydrochloride (3.4 g, 36.2 mmol), and N-hydroxybenzotriazole (4.8 g, 34.8 mmol) were dissolved in THF (32 mL) and cooled to 0° C. under N2. Diisopropylethylamine (12.3 mL, 71.3 mmol) was slowly added, maintaining the temperature below 25° C. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (7.0 g, 36.2 mmol) was then added slowly, maintaining the temperature at 15-25° C. The slurry was stirred overnight at room temperature, and then cooled to 0° C. 1N Aqueous HCl (36 mL) was added slowly, and the mixture was warmed to room temperature and extracted with EtOAc. The combined organic layers were washed with 10% aqueous HCl, saturated aqueous NaHCO3, and brine, and then concentrated and purified by silica gel chromatography to give the title compound.

1. temperaturemaintaining the temperature below 25° C
2. temperaturemaintaining the temperature at 15-25° C
3. temperaturecooled to 0° C
4. temperaturethe mixture was warmed to room temperature
5. extractionextracted with EtOAc
6. washThe combined organic layers were washed with 10% aqueous HCl, saturated aqueous NaHCO3, and brine
7. concentrationconcentrated
8. custompurified by silica gel chromatography