Phosphate → Nadh

Participants

Reaction Conditions

Reaction Steps

NAD+ (34.0 mg, 38.0 μmol) was placed in 2 mL of a 0.1 M phosphate buffer containing 0.07 M sodium iodide. The pH of this solution was adjusted to 8.1 with NaOH and then placed in an 80 mL Fisher-Porter bottle. The Fisber-Porter bottle was evacuated and refilled with argon three times then [Cl2Ru(TPPTS)2]2 (7.7 mg, 2.9 μmol) was added under a flow of argon. The Fisher-Porter bottle was evacuated and filled with 70 psi of H2 and stirred to 23° C. for 13.0 hr. The volatiles were removed under reduced pressure and a sample dissolved in D2O. To this was added 20 μl of trimethylsilylpropionate standard solution. 1H NMR integration shows that 7.4 μmole of NADH have been produced under these conditions.

Provenance & Evidence