beta-Maltose → alpha-Maltotriose

Participants

Reaction Steps

A portion of the amylase produced in Example I containing 90,000 amylase units/ml. was diluted 1/500 with 0.05 molar glycine buffer solution, and 80 ml. of the resulting solution were added to 250 ml. of an aqueous solution containing 0.0909 percent (weight/volume basis) amylose in 0.05 molar glycine buffer adjusted to pH 9.2 at 50° C. After 100 hours at 50° C. there was an apparent conversion of 75.5 percent of the amylose to maltose and a conversion of 4.6 percent of the amylose to glucose. A small quantity of maltotriose was also produced. Such analyses were carried out by well known procedures.

Provenance & Evidence