HCID 131711006, compound → HCID 131711005, compound

Participants

Reaction Steps

Each of the cis-and trans-isomers of tert-butyl 3-(2'-cyanovinyl)-7-phenoxyacetamido-3-cephem-4-carboxylate from Example 17 was in turn treated with 98-100 percent formic acid for 1 hour at room temperature to cleave the tert-butyl ester groups therefrom. After evaporation under reduced pressure of the excess formic acid, the acid residue was separated as an oil. Each product [the cis-or trans-isomer of the titled compound] showed a large antibiotic activity zone at 1 microgram/milliliter on a bioautograph test against B. subtilis.

Provenance & Evidence