HuagongsheHuagongshe Chemistry Print Report · For research reference only
L-Aspartic Acid → Pyruvic Acid
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | L-Aspartic Acid | HCID 5960 | — |
| Reactant | Oxaloacetate Ion | HCID 3260017 | — |
| Reactant | Magnesium Chloride | HCID 5360315 | — |
| Reactant | 3-(1H-Indol-3-yl)-2-oxopropanoate | HCID 5460337 | — |
| Product | Pyruvic Acid | HCID 1060 | — |
| Product | L-Tryptophan | HCID 6305 | — |
Reaction Steps
A solution of indole-3-pyruvate, 20 mM; L-aspartic acid 20 mM; MgCl2, 1.5 mM; transaminase, 0.3 mg/ml; oxaloacetate decarboxylase, 0.3 mg/ml buffered to pH 7.0 with 5 mM tris-hydroxymethylaminomethane hydrochloride (Tris) was stirred slowly for 2 hours. At the end of this time the reaction is complete. The L-tryptophan and pyruvic acid produced can be purified by methods well known in the art.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US04518692 |
| Source dataset | uspto-grants-1985_05 |
| ORD ID | ord-d77010d4b6754d73bea1dc6367e80517 |
| Source note | uspto-grants-1985_05 |