Acetate → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Participants

Reaction Steps

The end 15 of the electrode is placed into a solution of water soluble DCC (25 mg/ml) in acetate buffer, pH 4.5 for 1 hour. It is then rinsed, in buffer only, for 5 minutes and thereafter placed in a solution of glucose oxidase (10 mg/ml) in acetate buffer, ph 5.5, for 11/2 hours before again rinsing in buffer. The tip of the electrode 15, with the layers of dimethyl ferrocene and immobilised enzyme is then dipped into a solution of cellulose acetate dissolved in acetone and formamide and put into ice water for several minutes, to give a protected and stable electrode.

Provenance & Evidence