Hydrochloric Acid → HCID 131714389, compound

Participants

Reaction Steps

A mixture of 7-[2-methoxyimino-2-(2-formamidothiazol-4-yl)acetamido]-3-(3-formamido-1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (4.4 g) and conc. hydrochloric acid (1.6 ml) in methanol was stirred for 4 hours at room temperature and evaporated to dryness. The residue was dissolved in water (100 ml) and subjected to column chromatography on a non ionic adsorption resin "Diaion HP 20" (160 ml). After the column was washed with water, the elution was carried out with 20% aqueous methanol. The fractions containing an object compound were collected, evaporated to remove methanol under reduced pressure and lyophilized to give 7-[2-methoxyimino-2-(2-aminothiazol-4-yl)acetamido]-3-(3-amino-1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (2.3 g), mp 185° to 189° C. (dec.).

Provenance & Evidence