Citrate → [(2S,3S,5R)-5-[2-amino-6-(cyclobutoxy)purin-9-yl]-3-azido-tetrahydrofuran-2-yl]methanol

関与物質

反応条件

反応手順

To 800 mL of an aqueous pH 6.0, 50 mM citrate buffer, prepared as described in Example 2c, was added 2-amino-6-(cyclobutoxy)-9H-purine (0.164 g, 0.8 mmol) and 3'-azido-3'-deoxythymidine (1.069 g, 4.0 mmol). Solution was achieved by heating the mixture at 50° C. with sonication. A sample was removed as a control. A 40 mL solution of trans-N-deoxyribosylase with an activity of 1500 units/mL was added. The reaction was heated at 50° C. Five days later 0.164 g, 0.8 mmol, of 2-amino-6-cyclobutoxypurine was added to the reaction. Ten days after the reaction was started, another 0.164 g, 0.8 mmol, of 2-amino-6-cyclobutoxypurine and 0.428 g, 1.6 mmol, of 3'-azido-3'-deoxythymidine were added to the reaction. Twenty-one days after the reaction was started, 0.164 g, 0.8 mmol, of 2-amino-6-cyclobutoxypurine was added to the reaction. The reaction was stopped after twenty-six days by heating to 80° C. to precipitate the enzyme. After chilling to 0° C. the mixture was filtered. The aqueous filtrate was extracted with ethyl acetate (3 times) to remove the product. The combined ethyl acetate fractions were dried with magnesium sulfate, filtered and the solvent removed in vacuo to give a foam. The foam was chromatographed on basic alumina grade 1 eluted with chloroform/methanol (95.5, v/v). The product containing fractions were combined and the solvent removed in vacuo to give the product as a foam, 0.334 g, in 32% yield.

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