Ninhydrin → 6-Carboxyfluorescein succinimidyl ester

Participants

Reaction Steps

The resin can be treated with 2 equivalents 6-FAM-NHS in 1% DIEA/DMF and stirred or shaken at ambient temperature overnight. When completed, the resin can be drained, washed thrice with DMF, thrice with (1% DCM and 1% methanol) and dried to provide an orange resin that was negative by ninhydrin test.

Provenance & Evidence