Ammonia → HCID 57989070, compound

Participants

Reaction Steps

35% Aqueous ammonia (5 mL) was added to (R)-tert-butyl 2-hydroxy-2-(4-hydroxy-2-oxo-2,3-dihydrobenzo[d]thiazol-7-yl)ethyl(2-(5-((4-(2,2,2-trifluoroacetyl)-1-oxa-4,9-diazaspiro[5.5]undecan-9-yl)methyl)thiophen-3-yl)ethyl)carbamate (Example 264, step c) (0.2 g). After 40 min at 20° C. the solution was concentrated to ˜1 mL and the slurry added to a C18 (10 g) cartridge washing with water (20 mL), then eluting the product with methanol. The fractions containing product were combined and evaporated in vacuo. The resulting solid was dissolved in acetonitrile and evaporated in vacuo. Yield 0.11 g.

Provenance & Evidence