反应详情

温度

30 °C

4.0 g of pig liver esterase produced by Sigma [PLE (27 kU/g), lyophilized product, product number: E30191 was dissolved in a 0.05 mol/L phosphate buffer adjusted to pH 6.5 (360 mL), and the solution was added with a solution of diethyl 2-benzyloxycarbonylamino-2-ethoxycarbonylsuccinate (4.0 g) dissolved in ethanol (40 mL). The mixture was stirred at 30° C. for 20 hours to obtain (R)-1-ethyl hydrogen 3-benzyloxycarbonylamino-3-ethoxycarbonylsuccinate at a yield of 84% and optical purity of 93.6% ee.

1. custom4.0 g of pig liver esterase produced by Sigma [PLE (27 kU/g)