Ammonia → HCID 58577901, compound

Participants

Reaction Steps

Methyl 3-(5-(4-chloro-5-oxo-7,8-dihydropyrimido[5,4-f][1,4]oxazepin-6(5H)-yl)pyridin-2-yloxy)-2,2-dimethylpropanoate B-20 was dissolved in a 0.5 M solution of ammonia in 1,4-dioxane and stirred at room temperature for 20 h. The reaction mixture was then concentrated to dryness under reduced pressure. The residue was purified by flash column chromatography on silica gel (7 N ammonia solution in methanol:CH2Cl2 gradient) to give methyl 3-(5-(4-amino-5-oxo-7,8-dihydropyrimido[5,4-f][1,4]oxazepin-6(5H)-yl)pyridin-2-yloxy)-2,2-dimethylpropanoate B-21 (55.0 mg, Yield=57% from B-19). MS (ESI) [M+1]+ 388.

Provenance & Evidence