反应详情

To a solution of 50 mg (0.14 mmol) of mitomycin A in 10 ml of anhydrous methanol was stirred with 150 mg (2 mmol) of N-methylpropargylamine at room temperature. The reaction appeared to be complete in 3 hours, as revealed by TLC. The solvent was removed by evaporation under reduced pressure and the residue was chromatographed using silica-gel as adsorbent. The fraction obtained by eluting the column with ethyl acetate was evaporated under reduced pressure. Recrystallization from a mixture of methylene chloride and hexane gave 20 mg (37% yield) of the desired product as purple-colored crystals having a melting point of 86°-87° C. (decomposing) and providing the following analysis:

1. customThe solvent was removed by evaporation under reduced pressure
2. customthe residue was chromatographed
3. customThe fraction obtained
4. washby eluting the column with ethyl acetate
5. customwas evaporated under reduced pressure
6. customRecrystallization
7. additionfrom a mixture of methylene chloride and hexane