反应详情

温度

-18 °C

0.52 cm3 of triethylamine is added to a stirred mixture of 1 g of Boc-D-4-pyridylalanine in 10 cm3 of tetrahydrofuran, under an inert atmosphere, and the mixture is then cooled to a temperature in the region of −18° C. After addition of 0.47 cm3 of isobutyl chloroformate, stirring is continued for 30 minutes at a temperature of between −18° C. and −10° C. The mixture is rapidly filtered, followed by addition to the filtrate of 0.28 g of sodium borohydride predissolved in 2 cm3 of water. The mixture is stirred for 1 hour at a temperature in the region of 0° C. and then for 16 hours at a temperature in the region of 20° C. The mixture is made alkaline by addition of aqueous potassium carbonate solution and then stirred in the presence of ethyl acetate. The organic phase is separated out after settling has taken place and then washed with water, dried over magnesium sulfate, filtered and concentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C. The residue obtained is purified by chromatography under an argon pressure of 50 kPa on a column of silica gel (particle size 40-63μ: diameter 2.2 cm; height of silica 30 cm), eluting first with ethyl acetate alone and collecting 30-cm3 fractions. Fractions 1 to 18 are discarded and the column is then eluted with a mixture of ethyl acetate/methanol (90/10 by volume). Fractions 23 to 26 are collected and then combined. After concentration under reduced pressure (5 kPa) at a temperature in the region of 40° C., 0.10 g of tert-butyl (1R)-1-(4-pyridylmethyl)-2-hydroxy-ethylcarbamate is obtained in the form of an oil. Infrared spectrum (CH2Cl2): 3618; 3434; 2981; 1708; 1501; 1367; 1168 and 1057 cm−1.

1. filtrationThe mixture is rapidly filtered
2. additionfollowed by addition to the filtrate of 0.28 g of sodium borohydride
3. stirringThe mixture is stirred for 1 hour at a temperature in the region of 0° C.
4. waitfor 16 hours at a temperature in the region of 20° C
5. additionby addition of aqueous potassium carbonate solution
6. stirringstirred in the presence of ethyl acetate
7. customThe organic phase is separated out after settling
8. washwashed with water
9. dry with materialdried over magnesium sulfate
10. filtrationfiltered
11. concentrationconcentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C
12. customThe residue obtained
13. customis purified by chromatography under an argon pressure of 50 kPa on a column of silica gel (particle size 40-63μ: diameter 2.2 cm; height of silica 30 cm)
14. washeluting first with ethyl acetate alone
15. customcollecting 30-cm3 fractions
16. washthe column is then eluted with a mixture of ethyl acetate/methanol (90/10 by volume)
17. customFractions 23 to 26 are collected
18. concentrationAfter concentration under reduced pressure (5 kPa) at a temperature in the region of 40° C.